Then, 10 µl of supernatant was calculated using the Qubit 4 (ThermoFisher) dsDNA HS. The cfDNA concentration was calculated by normalizing the Qubit worth in relation to the volume of cells. aDNA is usually treated with a combination of uracil deglycosylase and endonuclease VIII (consumer remedy) to excise uracils and eradicate the C to T sign s